Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 127
... proton from either group thus produces a red shift of the absorption spectrum above 240 mp , but at shorter wavelengths , dissociation of a proton from the carboxyl group produces a blue shift , and from the amino group , a red shift ...
... proton from either group thus produces a red shift of the absorption spectrum above 240 mp , but at shorter wavelengths , dissociation of a proton from the carboxyl group produces a blue shift , and from the amino group , a red shift ...
Page 317
... protons so that protons can jump from one basic group to another . The fluctuations in proton density give rise to an asymmetric charge distri- bution and create a nonvanishing mean - square electric moment even if the mean permanent ...
... protons so that protons can jump from one basic group to another . The fluctuations in proton density give rise to an asymmetric charge distri- bution and create a nonvanishing mean - square electric moment even if the mean permanent ...
Page 320
... proton fluctuation . This high - frequency relax- ation process will be further discussed in the next section . The essence of Scheider's treatment lies in the conclusion that the relaxation proc- ess due to proton fluctuation should be ...
... proton fluctuation . This high - frequency relax- ation process will be further discussed in the next section . The essence of Scheider's treatment lies in the conclusion that the relaxation proc- ess due to proton fluctuation should be ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone