Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 15
... recording micrographs , since particles of interest are obscured by aggregations of material . This results in some ... recorded on a photographic plate . As power supply regulation de- teriorates , the paraxial regions of the recorded ...
... recording micrographs , since particles of interest are obscured by aggregations of material . This results in some ... recorded on a photographic plate . As power supply regulation de- teriorates , the paraxial regions of the recorded ...
Page 200
... recorded . The corresponding E ( X ) curve is divided into the apparent spectrum to yield the corrected curve ( e.g. , Fig ... recording of the fluorescence from a rhodamine B screen observed at 620 mμ while varying the excitation mono ...
... recorded . The corresponding E ( X ) curve is divided into the apparent spectrum to yield the corrected curve ( e.g. , Fig ... recording of the fluorescence from a rhodamine B screen observed at 620 mμ while varying the excitation mono ...
Page 465
... recording of the derivative plot ( e.g. , Tracey , 1964 ) is obviously to be preferred , but manual differentiation has usually been employed ( e.g. , Winzor and Scheraga , 1963 ) . This entails calculation of the change in ...
... recording of the derivative plot ( e.g. , Tracey , 1964 ) is obviously to be preferred , but manual differentiation has usually been employed ( e.g. , Winzor and Scheraga , 1963 ) . This entails calculation of the change in ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone