Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 132
... reference solutions of protein . If this latter perturbant occupies proportionately more space in the neighborhood ... Reference Reference Ethylene glycol " 1.433115 4.4 0.072 110 0.047 2709 Glycerol 1.473525 5.2 0.060 90 ...
... reference solutions of protein . If this latter perturbant occupies proportionately more space in the neighborhood ... Reference Reference Ethylene glycol " 1.433115 4.4 0.072 110 0.047 2709 Glycerol 1.473525 5.2 0.060 90 ...
Page 160
... reference - reference baseline will reveal any deviations in the baseline resulting from changes in the energy received by the phototubes because of slit - width changes ( or dynode voltage changes ) produced by the absorption of the ...
... reference - reference baseline will reveal any deviations in the baseline resulting from changes in the energy received by the phototubes because of slit - width changes ( or dynode voltage changes ) produced by the absorption of the ...
Page 161
... Reference Solutions For convenience , the reference solution for spectrophotometric titra- tions should have all the titratable chromophores in one absorbing form ( see Section IV ) . For titrations of phenolic groups , a neutral pH ...
... Reference Solutions For convenience , the reference solution for spectrophotometric titra- tions should have all the titratable chromophores in one absorbing form ( see Section IV ) . For titrations of phenolic groups , a neutral pH ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone