Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 65
... residues related by symmetry . Part of the shaded residue lies outside the cell but an equivalent set of atoms appears within the cell 6.88 Å further along the chain . The shaded alanyl residue , -NH -CH ( CH3 ) —CO— , may be taken as ...
... residues related by symmetry . Part of the shaded residue lies outside the cell but an equivalent set of atoms appears within the cell 6.88 Å further along the chain . The shaded alanyl residue , -NH -CH ( CH3 ) —CO— , may be taken as ...
Page 221
Sydney J. Leach. greater quantum yields of tryptophyl compared to tyrosyl residues in proteins may stem in part from the greater quenching of tyrosyl residue fluorescence in polypeptides . In the random copolymer the tryptophyl residues ...
Sydney J. Leach. greater quantum yields of tryptophyl compared to tyrosyl residues in proteins may stem in part from the greater quenching of tyrosyl residue fluorescence in polypeptides . In the random copolymer the tryptophyl residues ...
Page 230
... residues can give very misleading results if the quantum yields of the groups vary widely . Steiner et al . ( 1964 ) have measured the effect of propylene glycol on N - acetyl tryptophanamide and on several proteins . Particularly note ...
... residues can give very misleading results if the quantum yields of the groups vary widely . Steiner et al . ( 1964 ) have measured the effect of propylene glycol on N - acetyl tryptophanamide and on several proteins . Particularly note ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone