Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 35
... rotation photograph study of the phycoerythrin molecule by H. S. Slayter ( 1965 ) demon- strated that any given degree of rotation could produce an apparently significant enhancement of contrast , depending on the particle chosen ...
... rotation photograph study of the phycoerythrin molecule by H. S. Slayter ( 1965 ) demon- strated that any given degree of rotation could produce an apparently significant enhancement of contrast , depending on the particle chosen ...
Page 66
... rotation of 90 ° ( = 360 / 4 ) Center of symmetry ( a ) 2 - fold Rotation axis ( b ) 2 - fold Screw axis ( c ) 4 - fold Screw axis ( d ) FIG . 6. Symmetry operations encountered in dealing with proteins . If a center of symmetry is ...
... rotation of 90 ° ( = 360 / 4 ) Center of symmetry ( a ) 2 - fold Rotation axis ( b ) 2 - fold Screw axis ( c ) 4 - fold Screw axis ( d ) FIG . 6. Symmetry operations encountered in dealing with proteins . If a center of symmetry is ...
Page 184
... rotation about one axis . This model may be applicable to many fluorescent protein conjugates . In the model of Gottlieb and Wahl , two rotational diffusion coefficients are required to describe the polarization of fluorescence . These ...
... rotation about one axis . This model may be applicable to many fluorescent protein conjugates . In the model of Gottlieb and Wahl , two rotational diffusion coefficients are required to describe the polarization of fluorescence . These ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone