Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 202
... curve , it is less well known that the type of grating ( or prism ) in the emission monochromator is equally ... scatter peak at 278 mμ , which could be subtracted by using a buffer blank . Scatter signals en- countered in obtaining ...
... curve , it is less well known that the type of grating ( or prism ) in the emission monochromator is equally ... scatter peak at 278 mμ , which could be subtracted by using a buffer blank . Scatter signals en- countered in obtaining ...
Page 203
... curve of one instrument for spectra obtained with another . This is * -D ( a ) * Slits Scatter Mirror I To detector ( b ) S FIG . 12. Placement of solid samples for front surface observation in a fluorometer . Fluorescence ( arbitrary ...
... curve of one instrument for spectra obtained with another . This is * -D ( a ) * Slits Scatter Mirror I To detector ( b ) S FIG . 12. Placement of solid samples for front surface observation in a fluorometer . Fluorescence ( arbitrary ...
Page 213
... curve is exponential if only a single fluorescent species is present . Nonexponential decay is indicative of the ... scattering solution ( Chen et al . , 1967 ) . The other channel of the oscilloscope is used to display signals from a " decay ...
... curve is exponential if only a single fluorescent species is present . Nonexponential decay is indicative of the ... scattering solution ( Chen et al . , 1967 ) . The other channel of the oscilloscope is used to display signals from a " decay ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
Operational Requirements for HighResolution Electron | 15 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis binding Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone