Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 122
... sedimentation velocity and equilibrium experiments , by light scattering or osmotic pressure measurements . The ... sedimentation coefficient of ovomucoid is essentially unchanged between pH 122 JOHN W. DONOVAN Perturbation Difference ...
... sedimentation velocity and equilibrium experiments , by light scattering or osmotic pressure measurements . The ... sedimentation coefficient of ovomucoid is essentially unchanged between pH 122 JOHN W. DONOVAN Perturbation Difference ...
Page 414
Sydney J. Leach. monomer in instantaneous equilibrium with its polymer of different sedimentation coefficient = nA An By ignoring spreading of the sedimenting boundary due to diffusion , Gilbert was able to obtain an analytical solution ...
Sydney J. Leach. monomer in instantaneous equilibrium with its polymer of different sedimentation coefficient = nA An By ignoring spreading of the sedimenting boundary due to diffusion , Gilbert was able to obtain an analytical solution ...
Page 452
... sedimentation coefficient as co → 0 diffusion coefficient of a solute gas constant weight - average elution volume and molecular weight , respectively , of a solute system concentration and elution volume , respectively , of solute ...
... sedimentation coefficient as co → 0 diffusion coefficient of a solute gas constant weight - average elution volume and molecular weight , respectively , of a solute system concentration and elution volume , respectively , of solute ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone