Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 371
... separation of different molecular forms of enzymes and other biologically important macromolecules and their subunits , separation of peptides for sequential studies on proteins , genetic anal- yses , and analysis of the hemoglobin ...
... separation of different molecular forms of enzymes and other biologically important macromolecules and their subunits , separation of peptides for sequential studies on proteins , genetic anal- yses , and analysis of the hemoglobin ...
Page 420
... separation of even two components is impossible . Only the front and rear boundaries of overlapping zones of different electrophoretic components are observed ( see Fig . 1 ) . Furthermore , the method is not suitable for analysis of ...
... separation of even two components is impossible . Only the front and rear boundaries of overlapping zones of different electrophoretic components are observed ( see Fig . 1 ) . Furthermore , the method is not suitable for analysis of ...
Page 426
... separation is sufficiently short for diffusion to be minimal , and sharp discrete spots or zones are obtained . High - voltage electrophoresis is a powerful method for the separation of peptides and is used extensively in amino acid ...
... separation is sufficiently short for diffusion to be minimal , and sharp discrete spots or zones are obtained . High - voltage electrophoresis is a powerful method for the separation of peptides and is used extensively in amino acid ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone