Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 21
... SHADOW CASTING III . The Enhancement of Contrast 1. Practical Techniques In the method of shadow casting , heavy atoms are evaporated in a vacuum in such a way that the resulting vapor is incident at an angle to the surface of the ...
... SHADOW CASTING III . The Enhancement of Contrast 1. Practical Techniques In the method of shadow casting , heavy atoms are evaporated in a vacuum in such a way that the resulting vapor is incident at an angle to the surface of the ...
Page 25
... shadow ratio , which is length of shadow cast by specimen height of specimen contour above substrate ( 4 ) In fact , the effective shadow ratio is always somewhat less than the geometrical ratio as a consequence of scattering of metal ...
... shadow ratio , which is length of shadow cast by specimen height of specimen contour above substrate ( 4 ) In fact , the effective shadow ratio is always somewhat less than the geometrical ratio as a consequence of scattering of metal ...
Page 43
Sydney J. Leach. 2. Measurement of Shadow - Cast Particles In preparations contrasted by the shadow - casting method , it is pos- sible to determine the heights of the shadowed particles from the lengths of the shadows cast if the effective ...
Sydney J. Leach. 2. Measurement of Shadow - Cast Particles In preparations contrasted by the shadow - casting method , it is pos- sible to determine the heights of the shadowed particles from the lengths of the shadows cast if the effective ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone