Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 44
... shown by the dotted line , while the very irregular outline of the metal cap and shadow is shown by a solid line . Since the deposition of metal may not be radially symmetrical , and since the density of metal after evaporation may not ...
... shown by the dotted line , while the very irregular outline of the metal cap and shadow is shown by a solid line . Since the deposition of metal may not be radially symmetrical , and since the density of metal after evaporation may not ...
Page 86
... shown in Fig . 17 and it will be seen that the heavy atom - heavy atom peaks show up much more clearly than in the ordinary synthesis in Fig . 16a . 2 If sufficient centrosymmetric projections are present , all three heavy atom ...
... shown in Fig . 17 and it will be seen that the heavy atom - heavy atom peaks show up much more clearly than in the ordinary synthesis in Fig . 16a . 2 If sufficient centrosymmetric projections are present , all three heavy atom ...
Page 395
... shown by interacting systems will be discussed in Section III , E . D. ELECTROPHORESIS AS A CRITERION OF PURITY The criteria of purity of simple organic compounds , for example melt- ing point and elementary analysis , are not ...
... shown by interacting systems will be discussed in Section III , E . D. ELECTROPHORESIS AS A CRITERION OF PURITY The criteria of purity of simple organic compounds , for example melt- ing point and elementary analysis , are not ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone