Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 26
... significant regular structures at some positions . Thus , an apparent substructure of a shadowed particle cannot be considered significant un- less it is clearly and reproducibly differentiable from the surrounding background . Any ...
... significant regular structures at some positions . Thus , an apparent substructure of a shadowed particle cannot be considered significant un- less it is clearly and reproducibly differentiable from the surrounding background . Any ...
Page 78
... significant errors are introduced into the finer details of the electron density map . D. SERIES TERMINATION ERRORS The errors that would be introduced by excessive artificial sharpening are very similar to those caused by an abrupt ...
... significant errors are introduced into the finer details of the electron density map . D. SERIES TERMINATION ERRORS The errors that would be introduced by excessive artificial sharpening are very similar to those caused by an abrupt ...
Page 234
... significant increase in tryptophan fluorescence affords con- firmatory evidence of this molecular transition . Two overlapping transitions have been distinguished in slightly alka- line solutions of pepsinogen ( Frattali et al . , 1965 ) ...
... significant increase in tryptophan fluorescence affords con- firmatory evidence of this molecular transition . Two overlapping transitions have been distinguished in slightly alka- line solutions of pepsinogen ( Frattali et al . , 1965 ) ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone