Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
From inside the book
Results 1-3 of 76
Page 51
... single - chain model illustrated in Fig . 12c , but again the evidence is equivocal . The lengths of the shadows cast in preparations of the polymers are those of single fibrinogen molecules . This result , however , could be produced ...
... single - chain model illustrated in Fig . 12c , but again the evidence is equivocal . The lengths of the shadows cast in preparations of the polymers are those of single fibrinogen molecules . This result , however , could be produced ...
Page 61
... single atom of say carbon , we would find that the scattered intensity close to the main beam was 36 times greater than that from a single electron . This is because the waves from the six electrons in the carbon atom would all arrive ...
... single atom of say carbon , we would find that the scattered intensity close to the main beam was 36 times greater than that from a single electron . This is because the waves from the six electrons in the carbon atom would all arrive ...
Page 248
... single , rapid change in a property which is influencing the reaction , such as the temperature , the pressure , the electric field strength , etc. , or a cyclic variation in one of the parameters mentioned . The single - step technique ...
... single , rapid change in a property which is influencing the reaction , such as the temperature , the pressure , the electric field strength , etc. , or a cyclic variation in one of the parameters mentioned . The single - step technique ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
64 other sections not shown
Other editions - View all
Common terms and phrases
absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone