Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 155
... slit width by a factor of two ( obtained by an increase in photomultiplier voltage ) results in a 4 - fold decrease ... slit width depend on the curvature , not the slope , of that portion of the absorption spectrum viewed by the slit ...
... slit width by a factor of two ( obtained by an increase in photomultiplier voltage ) results in a 4 - fold decrease ... slit width depend on the curvature , not the slope , of that portion of the absorption spectrum viewed by the slit ...
Page 156
... slit width . ( 3 ) Resolution may be compromised even further , by use of still larger slit widths , if necessary . Even if the difference spectrum recorded does not show its maximum height , it will normally still show the proper ...
... slit width . ( 3 ) Resolution may be compromised even further , by use of still larger slit widths , if necessary . Even if the difference spectrum recorded does not show its maximum height , it will normally still show the proper ...
Page 158
... slit width , the slits should be opened until some change is observed , then closed again slightly so as to recover the optimum slit width and optimum signal - to - noise ratio . The sample cell and reference cell should then be ...
... slit width , the slits should be opened until some change is observed , then closed again slightly so as to recover the optimum slit width and optimum signal - to - noise ratio . The sample cell and reference cell should then be ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone