Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 151
... solution must be used as a reference solution . There is one serious disadvantage of the method when used for tyrosine analysis . The absorption of ... solution . The use of 3. ULTRAVIOLET ABSORPTION 151 Determination in Acid Solution.
... solution must be used as a reference solution . There is one serious disadvantage of the method when used for tyrosine analysis . The absorption of ... solution . The use of 3. ULTRAVIOLET ABSORPTION 151 Determination in Acid Solution.
Page 160
... solution can often be conveniently altered by adding concentrated acid or base solution to the sample solution using a syringe microburet or a glass rod drawn to a fine tip . Except at ex- tremes of pH , the volume change so produced is ...
... solution can often be conveniently altered by adding concentrated acid or base solution to the sample solution using a syringe microburet or a glass rod drawn to a fine tip . Except at ex- tremes of pH , the volume change so produced is ...
Page 161
... Solutions For convenience , the reference solution for spectrophotometric titra- tions should have all the titratable chromophores in one absorbing form ( see Section IV ) . For titrations of phenolic groups , a neutral pH reference ...
... Solutions For convenience , the reference solution for spectrophotometric titra- tions should have all the titratable chromophores in one absorbing form ( see Section IV ) . For titrations of phenolic groups , a neutral pH reference ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone