Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 33
... specific staining methods for Ο.Ιμ FIG . 8. A preparation of RNA - polymerase and DNA which is positively stained by uranyl acetate . The small , spherical electron - dense polymerase molecules are seen to be attached , at intervals ...
... specific staining methods for Ο.Ιμ FIG . 8. A preparation of RNA - polymerase and DNA which is positively stained by uranyl acetate . The small , spherical electron - dense polymerase molecules are seen to be attached , at intervals ...
Page 34
... specific positive staining of proteins and other molecules in thin tissue sections , is an increasingly important technique . Materials which combine with antibodies or with specific chemical reagents can be localized in tissues , as ...
... specific positive staining of proteins and other molecules in thin tissue sections , is an increasingly important technique . Materials which combine with antibodies or with specific chemical reagents can be localized in tissues , as ...
Page 418
... specific complexing between pepsin and bovine serum albumin ( see also Section VI , B in Chapter 3 ) . Mixtures of the enzyme E and its macromolecular substrate S were subjected to electrophoretic analyses under conditions such that ...
... specific complexing between pepsin and bovine serum albumin ( see also Section VI , B in Chapter 3 ) . Mixtures of the enzyme E and its macromolecular substrate S were subjected to electrophoretic analyses under conditions such that ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone