Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 7
... specimen . This mechanism of contrast is in fact normally taken for granted . Absorption contrast is enhanced in stained specimens . To a more limited degree ... Specimen Specimen Lens 1. ELECTRON MICROSCOPY OF GLOBULAR PROTEINS 7.
... specimen . This mechanism of contrast is in fact normally taken for granted . Absorption contrast is enhanced in stained specimens . To a more limited degree ... Specimen Specimen Lens 1. ELECTRON MICROSCOPY OF GLOBULAR PROTEINS 7.
Page 25
... specimen vertical distance of evaporation source above specimen ( 3 ) In principle , this quantity should be equal to the effective shadow ratio , which is length of shadow cast by specimen height of specimen contour above substrate ( 4 ) ...
... specimen vertical distance of evaporation source above specimen ( 3 ) In principle , this quantity should be equal to the effective shadow ratio , which is length of shadow cast by specimen height of specimen contour above substrate ( 4 ) ...
Page 46
... Specimens An ideal calibration specimen contains regular spacings which are known precisely by means which are independent of electron microscopy , and which , ideally , should be comparable in size to the objects to be measured . The ...
... Specimens An ideal calibration specimen contains regular spacings which are known precisely by means which are independent of electron microscopy , and which , ideally , should be comparable in size to the objects to be measured . The ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone