Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 304
... theory described above is therefore referred to as the Debye theory . However , any case in which the Debye theory is applicable is an ex- ception rather than a rule . Generally , the dielectric dispersion curves and the loss factors ...
... theory described above is therefore referred to as the Debye theory . However , any case in which the Debye theory is applicable is an ex- ception rather than a rule . Generally , the dielectric dispersion curves and the loss factors ...
Page 332
... theory , using a spherical model to explain the large dielectric increment of poly- vinylbenzylpyridine bromide ( which is also a random coil polyelectro- lyte ) . The random coil form of poly - L - glutamic acid is , however , a highly ...
... theory , using a spherical model to explain the large dielectric increment of poly- vinylbenzylpyridine bromide ( which is also a random coil polyelectro- lyte ) . The random coil form of poly - L - glutamic acid is , however , a highly ...
Page 390
... theory has been tested experimentally by Longsworth ( 1945 ) , who was also the first to apply the theory to the electrophoresis of proteins ( Longsworth , 1947 ) . Application of the Dole theory to pro- teins assumes that the pH and ...
... theory has been tested experimentally by Longsworth ( 1945 ) , who was also the first to apply the theory to the electrophoresis of proteins ( Longsworth , 1947 ) . Application of the Dole theory to pro- teins assumes that the pH and ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone