Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 113
... transition to an- other , resulting in an increase in absorption ( hyperchromism ) for a chromophore in one region ... transition dipoles . Figure 1 shows examples of two transition dipoles confined to a plane . In the first arrangement ...
... transition to an- other , resulting in an increase in absorption ( hyperchromism ) for a chromophore in one region ... transition dipoles . Figure 1 shows examples of two transition dipoles confined to a plane . In the first arrangement ...
Page 227
... transition or of the influence of charged carboxyl groups on tyrosyl fluorescence . A major increase in tyrosyl emission occurs in the acid transition of ribonuclease which runs parallel with optical rotation and difference absorption ...
... transition or of the influence of charged carboxyl groups on tyrosyl fluorescence . A major increase in tyrosyl emission occurs in the acid transition of ribonuclease which runs parallel with optical rotation and difference absorption ...
Page 234
... transition . Two overlapping transitions have been distinguished in slightly alka- line solutions of pepsinogen ( Frattali et al . , 1965 ) . Major changes in viscosity , optical rotation , and relaxation time accompany the second ...
... transition . Two overlapping transitions have been distinguished in slightly alka- line solutions of pepsinogen ( Frattali et al . , 1965 ) . Major changes in viscosity , optical rotation , and relaxation time accompany the second ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone