Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 24
... usually employed as a wire of 0.002 inch diameter ) . However , metals are often evaporated by means of resistance heating , from a refractory fila- ment usually made of tungsten , and in this case the form of the support- ing filament ...
... usually employed as a wire of 0.002 inch diameter ) . However , metals are often evaporated by means of resistance heating , from a refractory fila- ment usually made of tungsten , and in this case the form of the support- ing filament ...
Page 67
... usually be deduced by looking for regular gaps , called systematic absences , in the pattern of reflections obtained from the crystal . The space group is usually quoted in a sym- bolic form , for example C2 and P4,2,2 , which are ...
... usually be deduced by looking for regular gaps , called systematic absences , in the pattern of reflections obtained from the crystal . The space group is usually quoted in a sym- bolic form , for example C2 and P4,2,2 , which are ...
Page 159
... usually immedi- ately apparent from the magnitude of , or distortions in , the absorption spectra obtained . ( 3 ) When spectrophotometric titrations are being per- formed by measurement of either direct or difference absorption , non ...
... usually immedi- ately apparent from the magnitude of , or distortions in , the absorption spectra obtained . ( 3 ) When spectrophotometric titrations are being per- formed by measurement of either direct or difference absorption , non ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorbance absorption change absorption spectrum amino acids angle axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Chem chromophores coefficient concentration conformational changes contrast curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effect electric birefringence electric field electron microscope electrophoresis elution emission energy equation equilibrium excitation experimental factor film fluorescence frequency function gel filtration glycol instrument intensity interactions ionic strength ionization ions light macromolecules measured method mobility molar molecular weight molecules moving-boundary observed obtained optical parameter particles patterns peaks permanent dipole phase phenolic phenolic groups phenylalanine photomultiplier Phys polarization produced protein proton quantum yield ratio reaction relaxation residues ribonuclease rotational diffusion sample scattering shift shown in Fig solution solvent specimen spectra spectrofluorometer structure technique temperature theory tion tryptophan tyrosine ultraviolet unit cell values wavelength Weber zone