Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-18
... BamHI 27480 Hindl 26250 Pvul 26100 EcoRI 25160 Hindill 24780 Sacl 24510 Xbal 23130 Hindill 22430 Bgill 22350 BamHI 21230 EcoRI 19400 Smal 18560 Kpni 17050 Kpnl 11930 Pul 5510 BamHI M H ICLO E A || Z | U | V | G | T || KH54 rex cl nin5 ...
... BamHI 27480 Hindl 26250 Pvul 26100 EcoRI 25160 Hindill 24780 Sacl 24510 Xbal 23130 Hindill 22430 Bgill 22350 BamHI 21230 EcoRI 19400 Smal 18560 Kpni 17050 Kpnl 11930 Pul 5510 BamHI M H ICLO E A || Z | U | V | G | T || KH54 rex cl nin5 ...
Page 2-28
... BamHI 39890 Smal 39170 EcoRI 38810 Bgh 38750 Bgl 38100 Bgl 37460 Hindill 36900 Hindill 35790 Pvul 35710 Bgl 34500 BamHI 33500 Xhol 33240 Sall 32750 Sall 31750 EcoRI 31620 Smal 27980 BamHI 27480 Hindill 26250 Pvol 26100 EcoRI 25160 Hindi ...
... BamHI 39890 Smal 39170 EcoRI 38810 Bgh 38750 Bgl 38100 Bgl 37460 Hindill 36900 Hindill 35790 Pvul 35710 Bgl 34500 BamHI 33500 Xhol 33240 Sall 32750 Sall 31750 EcoRI 31620 Smal 27980 BamHI 27480 Hindill 26250 Pvol 26100 EcoRI 25160 Hindi ...
Page 2-44
... BamHI 39890 Smal 39170 EcoRI 38810 Bghl 38750 BgM 38100 Bgill 37460 Hindi 36900 Hindill 35790 Pvul 35710 Bg 34500 BamHI 33500 Xhol 33240 Sall 32750 San 31750 EcoRI 31620 Smal 27980 BamHI 27480 Hindill 26250 Pvul 26100 EcoRI 25160 Hindi ...
... BamHI 39890 Smal 39170 EcoRI 38810 Bghl 38750 BgM 38100 Bgill 37460 Hindi 36900 Hindill 35790 Pvul 35710 Bg 34500 BamHI 33500 Xhol 33240 Sall 32750 San 31750 EcoRI 31620 Smal 27980 BamHI 27480 Hindill 26250 Pvul 26100 EcoRI 25160 Hindi ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml