Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 79
Page vi
... Chapter 7 ; Brian Seed for permission to include a description of his unpublished procedure for screening libraries by recombination ( Chapter 10 ) and many other useful suggestions ; Doug Hanahan for advice on trans- formation ( Chapter ...
... Chapter 7 ; Brian Seed for permission to include a description of his unpublished procedure for screening libraries by recombination ( Chapter 10 ) and many other useful suggestions ; Doug Hanahan for advice on trans- formation ( Chapter ...
Page viii
... chapters on DNA sequencing and expression of cloned genes in mam- malian cells , respectively ; and to Judy Campbell , who contributed signifi- cantly to the preparation of the chapter on enzymes . We are also grateful to Mike Ockler ...
... chapters on DNA sequencing and expression of cloned genes in mam- malian cells , respectively ; and to Judy Campbell , who contributed signifi- cantly to the preparation of the chapter on enzymes . We are also grateful to Mike Ockler ...
Page 1-9
... Chapter 13 ) or for preparation of radiolabeled single - stranded probes ( see Chapter 10 ) . Plasmid Vectors Carrying Bacteriophage Promoters Many plasmid vectors carry promoters derived from bacteriophages T3 , T7 , and / or SP6 ...
... Chapter 13 ) or for preparation of radiolabeled single - stranded probes ( see Chapter 10 ) . Plasmid Vectors Carrying Bacteriophage Promoters Many plasmid vectors carry promoters derived from bacteriophages T3 , T7 , and / or SP6 ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
96 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml