Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 5-35
... polymerase I ( holoen- zyme ) , the large fragment of E. coli DNA polymerase I ( Klenow fragment ) , the DNA polymerases encoded by bacteriophages T4 and T7 , modified bac- teriophage T7 DNA polymerases ( Sequenase ... DNA POLYMERASES 5 35.
... polymerase I ( holoen- zyme ) , the large fragment of E. coli DNA polymerase I ( Klenow fragment ) , the DNA polymerases encoded by bacteriophages T4 and T7 , modified bac- teriophage T7 DNA polymerases ( Sequenase ... DNA POLYMERASES 5 35.
Page 5-44
... DNA polymerase ( M = 114,000 ) and the Klenow fragment of E. coli DNA polymerase I are similar in that each possesses a 5 ' - 3 ' polymerase activity and a 3'5 ' exonuclease activity that is more active on single - stranded DNA than on ...
... DNA polymerase ( M = 114,000 ) and the Klenow fragment of E. coli DNA polymerase I are similar in that each possesses a 5 ' - 3 ' polymerase activity and a 3'5 ' exonuclease activity that is more active on single - stranded DNA than on ...
Page 5-48
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Bacteriophage T7 DNA Polymerase ( Bacteriophage T7 - infected E. coli ) USES The DNA polymerase induced after infection of E. coli by bacteriophage T7 is a complex of two ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Bacteriophage T7 DNA Polymerase ( Bacteriophage T7 - infected E. coli ) USES The DNA polymerase induced after infection of E. coli by bacteriophage T7 is a complex of two ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml