Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-64
... Figure 1.9 shows the relationship between the size of a segment of DNA and the concentration required in a ligation mixture to achieve ji ratios of 0.5 , 1 , 2 , and 5 ( Dugaiczyk et al . 1975 ) . Consider now a ligation mixture that ...
... Figure 1.9 shows the relationship between the size of a segment of DNA and the concentration required in a ligation mixture to achieve ji ratios of 0.5 , 1 , 2 , and 5 ( Dugaiczyk et al . 1975 ) . Consider now a ligation mixture that ...
Page 1-65
... FIGURE 1.9 The relationship between the size of DNA and its concentration at four different j : i ratios . The figure ( modified from Dugaiczyk et al . 1975 ) shows graphs of the equation m.w. = 51.1 jli [ DNA ] 2 0.6 Fraction of vector ...
... FIGURE 1.9 The relationship between the size of DNA and its concentration at four different j : i ratios . The figure ( modified from Dugaiczyk et al . 1975 ) shows graphs of the equation m.w. = 51.1 jli [ DNA ] 2 0.6 Fraction of vector ...
Page 2-96
... Figure 2.7 ( top ) . ii . Use a piece of cardboard to cover about one quarter of each streak . Expose the remaining portion of each streak for 10 seconds to a hand - held ultraviolet light . Move the cardboard so that only half of each ...
... Figure 2.7 ( top ) . ii . Use a piece of cardboard to cover about one quarter of each streak . Expose the remaining portion of each streak for 10 seconds to a hand - held ultraviolet light . Move the cardboard so that only half of each ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml