Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-54
... HindIII . The resulting circular recombinant is then used to transform E. coli to ampicillin resistance . Because of the lack of com- plementarity between the HindIII and BamHI protruding ends , the vector fragment cannot circularize ...
... HindIII . The resulting circular recombinant is then used to transform E. coli to ampicillin resistance . Because of the lack of com- plementarity between the HindIII and BamHI protruding ends , the vector fragment cannot circularize ...
Page 2-35
... HindIII , and EcoRI ) and is used to clone large ( 10 kb to 22 kb ) DNA fragments . The polycloning - site sequences are inverted with respect to each other . Inserts that destroy the XhoI or BamHI site can be excised at flanking sites ...
... HindIII , and EcoRI ) and is used to clone large ( 10 kb to 22 kb ) DNA fragments . The polycloning - site sequences are inverted with respect to each other . Inserts that destroy the XhoI or BamHI site can be excised at flanking sites ...
Page 2-51
... HindIII , BamHI , and EcoRI sites are located in the 5 ' lacZ coding region . DNA fragments up to 9 kb in length can ... HindIII Insertion 24.7 18.1 0-9 Lac ( some Lac * ) BamHI + HindIII REFERENCES Meissner et al . ( 1987 ) ; M. Berman ...
... HindIII , BamHI , and EcoRI sites are located in the 5 ' lacZ coding region . DNA fragments up to 9 kb in length can ... HindIII Insertion 24.7 18.1 0-9 Lac ( some Lac * ) BamHI + HindIII REFERENCES Meissner et al . ( 1987 ) ; M. Berman ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml