Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 88
Page 2-70
Incubate overnight at 37 ° C with vigorous agitation ( 300 cycles / minute in a
rotary shaker ) . 2. Read the OD of the culture . Calculate the cell concentration
assuming that 1 OD 600 = 8 x 108 cells / ml . 600 3. Withdraw four aliquots , each
...
Incubate overnight at 37 ° C with vigorous agitation ( 300 cycles / minute in a
rotary shaker ) . 2. Read the OD of the culture . Calculate the cell concentration
assuming that 1 OD 600 = 8 x 108 cells / ml . 600 3. Withdraw four aliquots , each
...
Page 2-72
Incubate overnight at the appropriate temperature with vigorous agitation ( 300
cycles / minute in a rotary shaker ) . 2. Inoculate 500 ml of NZCYM , prewarmed to
37 ° C in each of four 2 - liter flasks , with 1 ml of the overnight culture per flask .
Incubate overnight at the appropriate temperature with vigorous agitation ( 300
cycles / minute in a rotary shaker ) . 2. Inoculate 500 ml of NZCYM , prewarmed to
37 ° C in each of four 2 - liter flasks , with 1 ml of the overnight culture per flask .
Page 4-48
Incubate for 1.0-1.5 hours at 37 ° C with strong agitation ( 300 cycles / minute ) .
The bacterial culture should be only slightly turbid at this stage . If growth is too
vigorous , dilute the culture with prewarmed 2x YT medium until the turbidity is
only ...
Incubate for 1.0-1.5 hours at 37 ° C with strong agitation ( 300 cycles / minute ) .
The bacterial culture should be only slightly turbid at this stage . If growth is too
vigorous , dilute the culture with prewarmed 2x YT medium until the turbidity is
only ...
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User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
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Common terms and phrases
activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies completely concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome growth Hindi host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations nucleic acids obtained original packaging particles pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes problem procedure promoter protein purified reaction recombinant Recover region remove replication restriction enzyme room temperature Sall segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume Xbal yield
References to this book
Bibliographic information
| Title | Molecular Cloning: A Laboratory Manual, Volume 1 Molecular Cloning: A Laboratory Manual, Joseph Sambrook |
| Authors | Joseph Sambrook, E. F. Fritsch, Tom Maniatis |
| Edition | 2 |
| Publisher | Cold Spring Harbor Laboratory, 1989 |
| Original from | the University of Michigan |
| Digitized | 23 Jul 2008 |
| ISBN | 0879693096, 9780879693091 |
| Length | 676 pages |
| Export Citation | BiBTeX EndNote RefMan |