Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 85
Page 1-101
... Incubate the filters for 30 minutes at 50 ° C . Important : Do not allow the filters to dry at any stage during the prewashing , prehybridization , or hybridization steps . Prewashing solution 5x SSC 0.5 % SDS 1 mM EDTA ( pH 8.0 ) 3 ...
... Incubate the filters for 30 minutes at 50 ° C . Important : Do not allow the filters to dry at any stage during the prewashing , prehybridization , or hybridization steps . Prewashing solution 5x SSC 0.5 % SDS 1 mM EDTA ( pH 8.0 ) 3 ...
Page 2-70
... Incubate overnight at 37 ° C with vigorous agitation ( 300 cycles / minute in a rotary shaker ) . 2. Read the OD 600 ... Incubate for 20 minutes at 37 ° C with intermittent shaking . 7. Add each infected aliquot to 500 ml of NZCYM ...
... Incubate overnight at 37 ° C with vigorous agitation ( 300 cycles / minute in a rotary shaker ) . 2. Read the OD 600 ... Incubate for 20 minutes at 37 ° C with intermittent shaking . 7. Add each infected aliquot to 500 ml of NZCYM ...
Page 2-72
... Incubate overnight at the appropriate temperature with vigorous agitation ( 300 cycles / minute in a rotary shaker ) ... Incubate at 37 ° C with vigorous shaking until the OD 600 of the cultures reaches 0.5 ( 3–4 hours ) . 3. Inoculate ...
... Incubate overnight at the appropriate temperature with vigorous agitation ( 300 cycles / minute in a rotary shaker ) ... Incubate at 37 ° C with vigorous shaking until the OD 600 of the cultures reaches 0.5 ( 3–4 hours ) . 3. Inoculate ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml