Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 55
Page 1-63
... products of ligation will be dimers and larger oligomers of the plasmid . A theoretical discussion of the effects of DNA concentration on the nature of ligation products is presented by Dugaiczyk et al . ( 1975 ; see also Bethesda Res ...
... products of ligation will be dimers and larger oligomers of the plasmid . A theoretical discussion of the effects of DNA concentration on the nature of ligation products is presented by Dugaiczyk et al . ( 1975 ; see also Bethesda Res ...
Page 1-70
... products . Intramolecular ligation is suppressed , and the ligation products are created exclusively by inter- molecular joining events . Thus , even at concentrations of DNA that favor circularization ( j : i = 10 ) , all the DNA products ...
... products . Intramolecular ligation is suppressed , and the ligation products are created exclusively by inter- molecular joining events . Thus , even at concentrations of DNA that favor circularization ( j : i = 10 ) , all the DNA products ...
Page 2-57
... product enhances the stability of the cII gene product , which in turn results in increased synthesis of the cI gene ... products to form enzymatically active proteins ( Beckwith 1964 ; Ullmann and Perrin 1970 ) . Bacterial gene encoding ...
... product enhances the stability of the cII gene product , which in turn results in increased synthesis of the cI gene ... products to form enzymatically active proteins ( Beckwith 1964 ; Ullmann and Perrin 1970 ) . Bacterial gene encoding ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
96 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml