Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-40
... Recover the precipitated nucleic acids by centrifugation at 10,000 rpm for 10 minutes at room temperature in a Sorvall SS34 rotor ( or its equivalent ) . 3. Decant the supernatant carefully , and invert the open tube to allow the last ...
... Recover the precipitated nucleic acids by centrifugation at 10,000 rpm for 10 minutes at room temperature in a Sorvall SS34 rotor ( or its equivalent ) . 3. Decant the supernatant carefully , and invert the open tube to allow the last ...
Page 7-17
... Recover the RNA by centrifugation at 10,000g for 30 minutes at 4 ° C . Carefully discard the supernatant . 10. Wash the pellet with 70 % ethanol , recentrifuge briefly , discard the supernatant , and allow the pellet of nucleic acid to ...
... Recover the RNA by centrifugation at 10,000g for 30 minutes at 4 ° C . Carefully discard the supernatant . 10. Wash the pellet with 70 % ethanol , recentrifuge briefly , discard the supernatant , and allow the pellet of nucleic acid to ...
Page 7-28
... Recover the poly ( A ) RNA by centrifugation at 10,000g for 15 minutes at 4 ° C . Carefully discard the supernatant , and wash the pellet ( which is often invisible ) with 70 % ethanol . Recentrifuge briefly , and allow the pellet of ...
... Recover the poly ( A ) RNA by centrifugation at 10,000g for 15 minutes at 4 ° C . Carefully discard the supernatant , and wash the pellet ( which is often invisible ) with 70 % ethanol . Recentrifuge briefly , and allow the pellet of ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml