Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 46
Page 2-26
... Smal 18560 Kpni 17050 Kppl 11930 Pvul 5510 BamHI KH54 rex cl nin5 D 46600 Rend 40790 Smal 39710 Bgill 39650 Bgill 39000 Bgfl 38770 Pvul 38690 Bgill 36970 Sall 35980 EcoRI 35970 Sacl -Smal 35960 Xbal -35950 Sall 35930 Hindill 33700 Sall ...
... Smal 18560 Kpni 17050 Kppl 11930 Pvul 5510 BamHI KH54 rex cl nin5 D 46600 Rend 40790 Smal 39710 Bgill 39650 Bgill 39000 Bgfl 38770 Pvul 38690 Bgill 36970 Sall 35980 EcoRI 35970 Sacl -Smal 35960 Xbal -35950 Sall 35930 Hindill 33700 Sall ...
Page 2-28
... Smal 27980 BamHI 27480 Hindill 26250 Pvol 26100 EcoRI 25160 Hindi 24780 Sack 24510 Xbal 23130 Hindill 22430 Bgl 22350 BamHI 21230 EcoRI 8- 19400 Smal 18560 Kpnl 17050 Kpnl 11930 Pvul 5510 BamHI 45770 Rend 39160 Smal 38880 Bgill 38820 ...
... Smal 27980 BamHI 27480 Hindill 26250 Pvol 26100 EcoRI 25160 Hindi 24780 Sack 24510 Xbal 23130 Hindill 22430 Bgl 22350 BamHI 21230 EcoRI 8- 19400 Smal 18560 Kpnl 17050 Kpnl 11930 Pvul 5510 BamHI 45770 Rend 39160 Smal 38880 Bgill 38820 ...
Page 2-50
... Smal 27980 BamHI 27480 Hindill 26250 Pvul 25100 EcoRI 25160 Hind 24780 Sact 24510 Xbal 23130 Hindlil 22430 Bgl 22350 BamHI 21230 EcoRI 19400 Smal 18560 Kpnl 17050 Kpnl 11930 Pvul 6510 BamHI | M | L | K |||| H ICLO E JAUZUVGT B 101 exo ...
... Smal 27980 BamHI 27480 Hindill 26250 Pvul 25100 EcoRI 25160 Hind 24780 Sact 24510 Xbal 23130 Hindlil 22430 Bgl 22350 BamHI 21230 EcoRI 19400 Smal 18560 Kpnl 17050 Kpnl 11930 Pvul 6510 BamHI | M | L | K |||| H ICLO E JAUZUVGT B 101 exo ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml