Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 5-3
... Table 5.1 ) carry modification ( methylation ) and ATP - dependent restriction ( cleavage ) activities in the same protein . Type III enzymes cut the DNA at the recognition site and then dissociate from the substrate . However , type I ...
... Table 5.1 ) carry modification ( methylation ) and ATP - dependent restriction ( cleavage ) activities in the same protein . Type III enzymes cut the DNA at the recognition site and then dissociate from the substrate . However , type I ...
Page 5-8
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Notes ( Table 5.1 ) Table supplied by R. Roberts . Recognition sequences are given using the standard abbreviations ( Nomenclature Committee of the International Union of ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Notes ( Table 5.1 ) Table supplied by R. Roberts . Recognition sequences are given using the standard abbreviations ( Nomenclature Committee of the International Union of ...
Page 5-35
... ( Table 5.8 ) Table 5.8 is modified from Kornberg and Kornberg ( 1974 ) and Lehman ( 1981 ) . TM " Sequenase is a derivative of bacteriophage T7 DNA polymerase that has been modified by chemical treatment ( Sequenase ) or by genetic ...
... ( Table 5.8 ) Table 5.8 is modified from Kornberg and Kornberg ( 1974 ) and Lehman ( 1981 ) . TM " Sequenase is a derivative of bacteriophage T7 DNA polymerase that has been modified by chemical treatment ( Sequenase ) or by genetic ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml