Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xix
... Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 AGAROSE CONCENTRATION 6.4 CONFORMATION OF THE DNA 6.5 APPLIED VOLTAGE 6.6 DIRECTION OF THE ELECTRIC FIELD 6.6 BASE COMPOSITION AND TEMPERATURE 6.6 PRESENCE OF INTERCALATING DYES 6.6 ...
... Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 AGAROSE CONCENTRATION 6.4 CONFORMATION OF THE DNA 6.5 APPLIED VOLTAGE 6.6 DIRECTION OF THE ELECTRIC FIELD 6.6 BASE COMPOSITION AND TEMPERATURE 6.6 PRESENCE OF INTERCALATING DYES 6.6 ...
Page 6-3
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Agarose Gel Electrophoresis Agarose , which is extracted from seaweed , is a linear polymer whose basic structure is но CH2OH но но D - galactose 3,6 - anhydro L ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Agarose Gel Electrophoresis Agarose , which is extracted from seaweed , is a linear polymer whose basic structure is но CH2OH но но D - galactose 3,6 - anhydro L ...
Page 6-9
... AGAROSE GELS Preparation of an Agarose Gel 1. Seal the edges of a clean , dry , glass plate ( or the open ends of the plastic tray supplied with the electrophoresis apparatus ) with autoclave tape so as to form a mold ( Figure 6.2 ) ...
... AGAROSE GELS Preparation of an Agarose Gel 1. Seal the edges of a clean , dry , glass plate ( or the open ends of the plastic tray supplied with the electrophoresis apparatus ) with autoclave tape so as to form a mold ( Figure 6.2 ) ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml