Molecular Cloning: A Laboratory Manual, Volume 1 |
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A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. 6 Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors Affecting the Rate of DNA Migration in Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 AGAROSE ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. 6 Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors Affecting the Rate of DNA Migration in Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 AGAROSE ...
Page 6-3
... discussed on the following pages . Factors Affecting the Rate of DNA Migration in Agarose Gels Gel Electrophoresis of DNA 6.3 Approximate pH Values for Various Concentrations of Stock Solutions B 3 Agarose Gel Electrophoresis 6 3.
... discussed on the following pages . Factors Affecting the Rate of DNA Migration in Agarose Gels Gel Electrophoresis of DNA 6.3 Approximate pH Values for Various Concentrations of Stock Solutions B 3 Agarose Gel Electrophoresis 6 3.
Page 6-9
... AGAROSE GELS Preparation of an Agarose Gel 1. Seal the edges of a clean , dry , glass plate ( or the open ends of the plastic tray supplied with the electrophoresis apparatus ) with ... Agarose Gel 6 9 STOCK SOLUTIONS AND BLOCKING AGENTS B 9.
... AGAROSE GELS Preparation of an Agarose Gel 1. Seal the edges of a clean , dry , glass plate ( or the open ends of the plastic tray supplied with the electrophoresis apparatus ) with ... Agarose Gel 6 9 STOCK SOLUTIONS AND BLOCKING AGENTS B 9.
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml