Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 79
Page 1-39
... Allow the rotor to stop without braking . If the bacterial debris does not form a tightly packed pellet , recentrifuge at 5000 rpm for a further 20 minutes , and then transfer as much of the supernatant as possible to a fresh bottle ...
... Allow the rotor to stop without braking . If the bacterial debris does not form a tightly packed pellet , recentrifuge at 5000 rpm for a further 20 minutes , and then transfer as much of the supernatant as possible to a fresh bottle ...
Page 4-32
... allow the last traces of supernatant to drain away from the precipitate . Watch carefully to make sure that the precipitate does not slide out of the tube . Wipe the neck of the tube with Kimwipes . 12. Allow the pellet to dry at room ...
... allow the last traces of supernatant to drain away from the precipitate . Watch carefully to make sure that the precipitate does not slide out of the tube . Wipe the neck of the tube with Kimwipes . 12. Allow the pellet to dry at room ...
Page 7-21
... allow any remaining fluid to drain into the pad of Kimwipes . Return the tube to an upright position . Check that the pellet of RNA has not fallen out of the tube . Depending on the amount of RNA present , the pellet may be invisible in ...
... allow any remaining fluid to drain into the pad of Kimwipes . Return the tube to an upright position . Check that the pellet of RNA has not fallen out of the tube . Depending on the amount of RNA present , the pellet may be invisible in ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml