Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 77
Page 1-60
... amount of CIP and incubate under the appro- priate conditions . 10x CIP dephosphorylation buffer 10 mм ZnCl2 10 mM MgCl2 100 mM Tris Cl ( pH 8.3 ) Amount of CIP required Incubation conditions Protruding 5 ' termini 1 unit / 100 pmoles ...
... amount of CIP and incubate under the appro- priate conditions . 10x CIP dephosphorylation buffer 10 mм ZnCl2 10 mM MgCl2 100 mM Tris Cl ( pH 8.3 ) Amount of CIP required Incubation conditions Protruding 5 ' termini 1 unit / 100 pmoles ...
Page 2-94
... amount of arms and different amounts of potential insert . The amounts of inserts recommended in Table 2.5 have been calculated on the assumption that the ligation mixtures will contain 1 μg of bacteriophage A arms , 40 kb in size . In ...
... amount of arms and different amounts of potential insert . The amounts of inserts recommended in Table 2.5 have been calculated on the assumption that the ligation mixtures will contain 1 μg of bacteriophage A arms , 40 kb in size . In ...
Page 4-35
... amount of vector DNA and an appropriate amount of a test DNA that has been successfully cloned into bacteriophage M13 on a previous occasion ( e.g. , bacteriophage A DNA cleaved with restriction enzymes that recognize tetranucleotide ...
... amount of vector DNA and an appropriate amount of a test DNA that has been successfully cloned into bacteriophage M13 on a previous occasion ( e.g. , bacteriophage A DNA cleaved with restriction enzymes that recognize tetranucleotide ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
96 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml