Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxvii
... Antibodies in Immunological Screening 12.11 Choosing the Antibody 12.11 Purification of Antisera 12.13 Methods Used to Detect Antibodies Bound to Proteins Expressed in E. coli 12.14 Validation of Clones Isolated by Immunological ...
... Antibodies in Immunological Screening 12.11 Choosing the Antibody 12.11 Purification of Antisera 12.13 Methods Used to Detect Antibodies Bound to Proteins Expressed in E. coli 12.14 Validation of Clones Isolated by Immunological ...
Page xxxiv
... Antibodies 18.7 Raising Antisera Against Synthetic Peptides 18.7 COUPLING OF SYNTHETIC PEPTIDES TO KEYHOLE LIMPET HEMOCYANIN 18.8 Collection and Storage of Antisera 18.10 Purification of Antibodies 18.11 PURIFICATION OF ANTIBODIES BY ...
... Antibodies 18.7 Raising Antisera Against Synthetic Peptides 18.7 COUPLING OF SYNTHETIC PEPTIDES TO KEYHOLE LIMPET HEMOCYANIN 18.8 Collection and Storage of Antisera 18.10 Purification of Antibodies 18.11 PURIFICATION OF ANTIBODIES BY ...
Page xxxv
... Antibody Complexes 18.42 PRECLEARING THE CELL LYSATE 18.43 Immunoprecipitation of the Target Protein 18.44 SDS ... Antibody to the Target Protein 18.70 INCUBATING THE NITROCELLULOSE FILTER WITH THE PRIMARY ANTIBODY DIRECTED AGAINST THE ...
... Antibody Complexes 18.42 PRECLEARING THE CELL LYSATE 18.43 Immunoprecipitation of the Target Protein 18.44 SDS ... Antibody to the Target Protein 18.70 INCUBATING THE NITROCELLULOSE FILTER WITH THE PRIMARY ANTIBODY DIRECTED AGAINST THE ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml