Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 29
Page xxxiii
... Assays for Chloramphenicol Acetyltransferase 16.60 METHOD 1 : THIN - LAYER CHROMATOGRAPHY 16.60 METHOD 2 : EXTRACTION WITH ORGANIC SOLVENTS 16.63 METHOD 3 : DIFFUSION OF REACTION PRODUCTS INTO SCINTILLATION FLUID 16.64 Assay for B ...
... Assays for Chloramphenicol Acetyltransferase 16.60 METHOD 1 : THIN - LAYER CHROMATOGRAPHY 16.60 METHOD 2 : EXTRACTION WITH ORGANIC SOLVENTS 16.63 METHOD 3 : DIFFUSION OF REACTION PRODUCTS INTO SCINTILLATION FLUID 16.64 Assay for B ...
Page 1-49
... assay by approximately 200 - fold . 1. Add sufficient water to reduce the concentration of ethidium bromide to < 0.5 mg / ml . 2. To the resulting solution , add 0.2 volume of fresh 5 % hypophosphorous acid and 0.12 volume of fresh 0.5 ...
... assay by approximately 200 - fold . 1. Add sufficient water to reduce the concentration of ethidium bromide to < 0.5 mg / ml . 2. To the resulting solution , add 0.2 volume of fresh 5 % hypophosphorous acid and 0.12 volume of fresh 0.5 ...
Page 2-61
... assayed into a sterile tube ( 13 mm x 100 mm ) . 3. Add 0.1 ml of plating bacteria to each tube . Mix by shaking or ... assay of bac- teriophage λ . During incubation at 37 ° C , droplets of moisture that form on the surface of the top ...
... assayed into a sterile tube ( 13 mm x 100 mm ) . 3. Add 0.1 ml of plating bacteria to each tube . Mix by shaking or ... assay of bac- teriophage λ . During incubation at 37 ° C , droplets of moisture that form on the surface of the top ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml