Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 73
Page 6-7
... buffer for electrophoresis of denatured single- stranded DNA is 50 mn NaOH , 1 mM EDTA ( alkaline electrophoresis buffer ; see Table 6.2 ) . As discussed below , agarose cannot be melted in the presence of NaOH . Therefore , the agarose ...
... buffer for electrophoresis of denatured single- stranded DNA is 50 mn NaOH , 1 mM EDTA ( alkaline electrophoresis buffer ; see Table 6.2 ) . As discussed below , agarose cannot be melted in the presence of NaOH . Therefore , the agarose ...
Page 7-27
... buffer . Apply the solution to the column , and immediately begin to collect the eluate in a sterile tube . When all of the RNA solution has entered the column , add 1 column volume of 1 × column - loading buffer and continue to collect ...
... buffer . Apply the solution to the column , and immediately begin to collect the eluate in a sterile tube . When all of the RNA solution has entered the column , add 1 column volume of 1 × column - loading buffer and continue to collect ...
Page 7-43
... buffer yellows with age if it is exposed to light or is autoclaved . Straw - colored buffer works well , but darker buffer does not . Caution : DEPC is suspected to be a carcinogen and should be handled with care . 2. Prepare the gel by ...
... buffer yellows with age if it is exposed to light or is autoclaved . Straw - colored buffer works well , but darker buffer does not . Caution : DEPC is suspected to be a carcinogen and should be handled with care . 2. Prepare the gel by ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml