Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-9
... Carrying Bacteriophage Promoters Many plasmid vectors carry promoters derived from bacteriophages T3 , T7 , and / or SP6 adjacent to the polycloning site . Foreign DNAs inserted at restriction sites can therefore be transcribed in vitro ...
... Carrying Bacteriophage Promoters Many plasmid vectors carry promoters derived from bacteriophages T3 , T7 , and / or SP6 adjacent to the polycloning site . Foreign DNAs inserted at restriction sites can therefore be transcribed in vitro ...
Page 1-54
... carry tandem copies of foreign DNA restriction sites at junctions between plasmid and foreign DNAs are usually conserved background of nonrecombinant clones is low foreign DNA is inserted in only one orientation within recombinant ...
... carry tandem copies of foreign DNA restriction sites at junctions between plasmid and foreign DNAs are usually conserved background of nonrecombinant clones is low foreign DNA is inserted in only one orientation within recombinant ...
Page 2-13
... carry repeated sequences ( see , e.g. , Lauer et al . 1980 ) . To avoid this problem , several vectors have been designed that carry the gam gene on one of the arms of the bacteriophage DNA . Examples of such vectors are Asep6 - lac5 ...
... carry repeated sequences ( see , e.g. , Lauer et al . 1980 ) . To avoid this problem , several vectors have been designed that carry the gam gene on one of the arms of the bacteriophage DNA . Examples of such vectors are Asep6 - lac5 ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml