Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 84
Page 1-75
... cells ( Neumann et al . 1982 ; Potter et al . 1984 ; Fromm et al . 1985 ; Chu et al . 1987 ) , but it has recently been used to transform E. coli ( Dower et al . 1988 ; Taketo 1988 ) and other bacteria ( Chassy and Flickinger 1987 ...
... cells ( Neumann et al . 1982 ; Potter et al . 1984 ; Fromm et al . 1985 ; Chu et al . 1987 ) , but it has recently been used to transform E. coli ( Dower et al . 1988 ; Taketo 1988 ) and other bacteria ( Chassy and Flickinger 1987 ...
Page 1-80
... cells from the −70 ° C freezer . Thaw the cells by holding the tube in the palm of your hand . Just as the cells thaw , transfer the tube to an ice bath . Store the cells on ice for 10 minutes . v . Using a chilled , sterile pipette ...
... cells from the −70 ° C freezer . Thaw the cells by holding the tube in the palm of your hand . Just as the cells thaw , transfer the tube to an ice bath . Store the cells on ice for 10 minutes . v . Using a chilled , sterile pipette ...
Page 7-12
... Cells This is a convenient and well - tested procedure for purifying cytoplasmic RNA from cells grown in tissue culture . The method is similar to that described previously to isolate total cellular RNA , except that the nuclei are ...
... Cells This is a convenient and well - tested procedure for purifying cytoplasmic RNA from cells grown in tissue culture . The method is similar to that described previously to isolate total cellular RNA , except that the nuclei are ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
96 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml