Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-39
... centrifugation at 5000 rpm for 15 minutes at room temperature in a Sorvall GS3 rotor ( or its equivalent ) . Salt may precipitate if centrifugation is carried out at 4 ° C . 8. Decant the supernatant carefully , and invert the open ...
... centrifugation at 5000 rpm for 15 minutes at room temperature in a Sorvall GS3 rotor ( or its equivalent ) . Salt may precipitate if centrifugation is carried out at 4 ° C . 8. Decant the supernatant carefully , and invert the open ...
Page 1-51
... centrifugation in CsCl - ethidium bromide gradients is small , the number of RNA molecules can be relatively large and can contribute significantly to the total number of 5 ' termini in a restriction digest . RNA can be removed from ...
... centrifugation in CsCl - ethidium bromide gradients is small , the number of RNA molecules can be relatively large and can contribute significantly to the total number of 5 ' termini in a restriction digest . RNA can be removed from ...
Page 7-19
... centrifugation , the RNA forms a pellet on the bottom of the tube , while the DNA and protein float in the supernatant solution . Small RNAs ( e.g. , 5S RNA and tRNAs ) , which do not sediment efficiently during centrifugation through ...
... centrifugation , the RNA forms a pellet on the bottom of the tube , while the DNA and protein float in the supernatant solution . Small RNAs ( e.g. , 5S RNA and tRNAs ) , which do not sediment efficiently during centrifugation through ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml