Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-90
... colonies carrying cosmids or plasmids that contain DNA sequences of interest . Methods are presented below to screen bacterial colonies with radiolabeled probes that are longer than 100 nucleotides in length . The preparation of these ...
... colonies carrying cosmids or plasmids that contain DNA sequences of interest . Methods are presented below to screen bacterial colonies with radiolabeled probes that are longer than 100 nucleotides in length . The preparation of these ...
Page 1-93
... colonies simultaneously from the surfaces of agar plates to nitrocellulose filters . This method works with bacterial colonies of any size , but small colonies ( 0.1-0.2 mm ) give the best results ; they produce sharper hybridization ...
... colonies simultaneously from the surfaces of agar plates to nitrocellulose filters . This method works with bacterial colonies of any size , but small colonies ( 0.1-0.2 mm ) give the best results ; they produce sharper hybridization ...
Page 1-93
... colonies simultaneously from the surfaces of agar plates to nitrocellulose filters . This method works with bacterial colonies of any size , but small colonies ( 0.1-0.2 mm ) give the best results ; they produce sharper hybridization ...
... colonies simultaneously from the surfaces of agar plates to nitrocellulose filters . This method works with bacterial colonies of any size , but small colonies ( 0.1-0.2 mm ) give the best results ; they produce sharper hybridization ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml