Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 80
Page 1-63
... concentration of compatible DNA termini . This is true no matter whether the termini are located on the same molecule of DNA ( intramolecular ligation ) or on different molecules ( intermolecular ligation ) . Consider the simple case of ...
... concentration of compatible DNA termini . This is true no matter whether the termini are located on the same molecule of DNA ( intramolecular ligation ) or on different molecules ( intermolecular ligation ) . Consider the simple case of ...
Page 1-64
... concentration of the DNA . Theoretically , when j = i , the end of a given DNA molecule is equally likely to make contact either with the other end of the same molecule or the end of a different molecule . Thus , under these conditions ...
... concentration of the DNA . Theoretically , when j = i , the end of a given DNA molecule is equally likely to make contact either with the other end of the same molecule or the end of a different molecule . Thus , under these conditions ...
Page 7-14
... concentration of 1000 units / ml or 10 mм , respectively . 12. Add RNAase - free pancreatic DNAase I ( see Appendix B ) to a final concentration of 2 μg / ml . Incubate for 60 minutes at 37 ° C . 13. Add EDTA and SDS to final concentrations ...
... concentration of 1000 units / ml or 10 mм , respectively . 12. Add RNAase - free pancreatic DNAase I ( see Appendix B ) to a final concentration of 2 μg / ml . Incubate for 60 minutes at 37 ° C . 13. Add EDTA and SDS to final concentrations ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml