Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 4-22
... ( containing , e.g. , agar or agarose ) , the diffusion of the progeny particles is limited . Filamentous bacteriophages such as M13 do not kill the cells they infect , but they do slow their growth approximately twofold . Under these ...
... ( containing , e.g. , agar or agarose ) , the diffusion of the progeny particles is limited . Filamentous bacteriophages such as M13 do not kill the cells they infect , but they do slow their growth approximately twofold . Under these ...
Page 6-32
... containing 0.6 M NaCl , and gently resuspend the resin . Allow the resin to settle once more , and then remove most ... containing a small circle of filter paper to retain the DEAE- Sephacel can also be used . 3. Wash the column with ...
... containing 0.6 M NaCl , and gently resuspend the resin . Allow the resin to settle once more , and then remove most ... containing a small circle of filter paper to retain the DEAE- Sephacel can also be used . 3. Wash the column with ...
Page 7-43
... Containing Formaldehyde This method is adapted from those of Lehrach et al . ( 1977 ) , Goldberg ( 1980 ) , and Seed ( 1982a ) . Caution : Formaldehyde vapors are toxic . Solutions containing formal- dehyde should be prepared in a ...
... Containing Formaldehyde This method is adapted from those of Lehrach et al . ( 1977 ) , Goldberg ( 1980 ) , and Seed ( 1982a ) . Caution : Formaldehyde vapors are toxic . Solutions containing formal- dehyde should be prepared in a ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml