Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 3-5
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Cloning in Cosmid Vectors In their simplest form , cosmid vectors are modified plasmids that carry a copy of the DNA sequences ( cos sequences ) required for packaging DNA ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Cloning in Cosmid Vectors In their simplest form , cosmid vectors are modified plasmids that carry a copy of the DNA sequences ( cos sequences ) required for packaging DNA ...
Page 3-18
... cosmids that carry selectable markers for eukaryotic cells are de- scribed on the following pages . pHC79-2cos / tk pHC79-2cos / tk ( Lindenmaier et al 3.18 Cosmid Vectors COSMID VECTORS FOR TRANSFECTION OF MAMMALIAN CELLS 3 18.
... cosmids that carry selectable markers for eukaryotic cells are de- scribed on the following pages . pHC79-2cos / tk pHC79-2cos / tk ( Lindenmaier et al 3.18 Cosmid Vectors COSMID VECTORS FOR TRANSFECTION OF MAMMALIAN CELLS 3 18.
Page 3-52
... Cosmids Because circular cosmid molecules carried in bacterial cells contain a cos site , they can be efficiently packaged into bacteriophage A particles . When cosmid - containing bacteria are infected with bacteriophage ( or when a ...
... Cosmids Because circular cosmid molecules carried in bacterial cells contain a cos site , they can be efficiently packaged into bacteriophage A particles . When cosmid - containing bacteria are infected with bacteriophage ( or when a ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml