Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 3-5
... vector DNA in vitro under conditions that favor the formation of the type of structure shown in Figure 3.1 . Here , the segment of foreign DNA is flanked by cosmid molecules and the two cos sites are arranged in the ... Cosmid Vectors 3.5.
... vector DNA in vitro under conditions that favor the formation of the type of structure shown in Figure 3.1 . Here , the segment of foreign DNA is flanked by cosmid molecules and the two cos sites are arranged in the ... Cosmid Vectors 3.5.
Page 3-7
... vector ( Bates and Swift 1983 ; Poustka et al . 1984 ) . As discussed later , the presence of these two sites greatly simplifies directional cloning in cosmid vectors ( see Figure 3.3 ) . • The construction of cosmid ... Cosmid Vectors 3.7.
... vector ( Bates and Swift 1983 ; Poustka et al . 1984 ) . As discussed later , the presence of these two sites greatly simplifies directional cloning in cosmid vectors ( see Figure 3.3 ) . • The construction of cosmid ... Cosmid Vectors 3.7.
Page 3-35
... vector . The principle of the method is shown in Figure 3.2 . In two separate aliquots , the cosmid vector is digested with restriction enzymes ... COSMID VECTORS DIGESTED WITH TWO RESTRICTION ENZYMES AND TREATED WITH PHOSPHATASE 3 35.
... vector . The principle of the method is shown in Figure 3.2 . In two separate aliquots , the cosmid vector is digested with restriction enzymes ... COSMID VECTORS DIGESTED WITH TWO RESTRICTION ENZYMES AND TREATED WITH PHOSPHATASE 3 35.
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml