Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 88
Page 133
However , the following procedure gives reproducibly high yields ( 2–5 mg of
plasmid DNA per 500 ml of culture ) with strains of E. coli harboring high - copy -
number plasmids carrying the pMB1 or ColE1 replicon . 1. Grow a 30 - ml culture
of ...
However , the following procedure gives reproducibly high yields ( 2–5 mg of
plasmid DNA per 500 ml of culture ) with strains of E. coli harboring high - copy -
number plasmids carrying the pMB1 or ColE1 replicon . 1. Grow a 30 - ml culture
of ...
Page 2-70
In our hands , however , the following protocol for the preparation of
bacteriophage from a 2 - liter culture of infected bacteria gives the best results (
Blattner et al . 1977 ; Maniatis et al . 1978 ) . 1. Place 100 ml of NZCYM in a 500 -
ml flask and ...
In our hands , however , the following protocol for the preparation of
bacteriophage from a 2 - liter culture of infected bacteria gives the best results (
Blattner et al . 1977 ; Maniatis et al . 1978 ) . 1. Place 100 ml of NZCYM in a 500 -
ml flask and ...
Page 4-48
Suspend a fresh bacterial colony containing pUC118 or pUC119 or their
derivatives in a sterile 15 - ml culture tube containing 2–3 ml of 2 x YT medium .
Add M13K07 to a final concentration of 2 x 10 ' pfu / ml . Incubate for 1.0-1.5
hours at 37 ...
Suspend a fresh bacterial colony containing pUC118 or pUC119 or their
derivatives in a sterile 15 - ml culture tube containing 2–3 ml of 2 x YT medium .
Add M13K07 to a final concentration of 2 x 10 ' pfu / ml . Incubate for 1.0-1.5
hours at 37 ...
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User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
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Common terms and phrases
activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies completely concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome growth Hindi host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations nucleic acids obtained original packaging particles pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes problem procedure promoter protein purified reaction recombinant Recover region remove replication restriction enzyme room temperature Sall segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume Xbal yield
References to this book
Bibliographic information
| Title | Molecular Cloning: A Laboratory Manual, Volume 1 Molecular Cloning: A Laboratory Manual, Joseph Sambrook |
| Authors | Joseph Sambrook, E. F. Fritsch, Tom Maniatis |
| Edition | 2 |
| Publisher | Cold Spring Harbor Laboratory, 1989 |
| Original from | the University of Michigan |
| Digitized | 23 Jul 2008 |
| ISBN | 0879693096, 9780879693091 |
| Length | 676 pages |
| Export Citation | BiBTeX EndNote RefMan |