Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 6-44
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. DETECTION OF DNA IN POLYACRYLAMIDE GELS Staining with Ethidium Bromide Because polyacrylamide quenches the fluorescence of ethidium bromide , it is not possible to detect ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. DETECTION OF DNA IN POLYACRYLAMIDE GELS Staining with Ethidium Bromide Because polyacrylamide quenches the fluorescence of ethidium bromide , it is not possible to detect ...
Page 6-52
... detection of minor species of DNA stained with ethidium bromide , the gels are usually destained in water for extended periods of time ( 24-48 hours ) before photography . For details of methods that can be used to maximize the ...
... detection of minor species of DNA stained with ethidium bromide , the gels are usually destained in water for extended periods of time ( 24-48 hours ) before photography . For details of methods that can be used to maximize the ...
Page 7-84
... detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl - paper and hy- bridization with DNA probes . Proc . Natl . Acad . Sci . 74 : 5350 . Alwine , J.C. , D.J. Kemp , B.A. Parker , J. Reiser , J. Renart , G.R. ...
... detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl - paper and hy- bridization with DNA probes . Proc . Natl . Acad . Sci . 74 : 5350 . Alwine , J.C. , D.J. Kemp , B.A. Parker , J. Reiser , J. Renart , G.R. ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml