Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 71
Page 6-3
... determined by the concentration of the agarose . When an electric field is applied across the gel , DNA , which is negatively charged at neutral pH , migrates toward the anode . The rate of migration is determined by a number of ...
... determined by the concentration of the agarose . When an electric field is applied across the gel , DNA , which is negatively charged at neutral pH , migrates toward the anode . The rate of migration is determined by a number of ...
Page 6-36
... determined by the length of the chains and the degree of cross - linking . H - CH2 = CH — C — N - CH2 - N — C — CH = CH2 N , N ' - methylenebisacrylamide H2 - CH - ICH2 CH2 - CH - CH2 - CH - ] , CH2 - CH— [ CH2 — CH — ] „ CH2— CO CO NH2 ...
... determined by the length of the chains and the degree of cross - linking . H - CH2 = CH — C — N - CH2 - N — C — CH = CH2 N , N ' - methylenebisacrylamide H2 - CH - ICH2 CH2 - CH - CH2 - CH - ] , CH2 - CH— [ CH2 — CH — ] „ CH2— CO CO NH2 ...
Page 7-75
... determined empirically by RNAase digestion of a series of hybridization mixtures containing different ratios of probe RNA to target RNA . If necessary , the system can be calibrated by setting up a series of control reactions containing ...
... determined empirically by RNAase digestion of a series of hybridization mixtures containing different ratios of probe RNA to target RNA . If necessary , the system can be calibrated by setting up a series of control reactions containing ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml