Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 84
Page 1-74
... efficiency of transformation of different bacterial strains by plasmids . Bac- teria treated according to the original protocol of Mandel and Higa yield 105–10 ° transformed colonies / μg of supercoiled plasmid DNA . This efficiency can ...
... efficiency of transformation of different bacterial strains by plasmids . Bac- teria treated according to the original protocol of Mandel and Higa yield 105–10 ° transformed colonies / μg of supercoiled plasmid DNA . This efficiency can ...
Page 1-75
... efficiencies . The efficiency of transformation with linear bacteriophage à DNA molecules is only 0.1 % of that obtained with small supercoiled plasmid DNA . A two- to tenfold variation in transforma- tion efficiency has been observed ...
... efficiencies . The efficiency of transformation with linear bacteriophage à DNA molecules is only 0.1 % of that obtained with small supercoiled plasmid DNA . A two- to tenfold variation in transforma- tion efficiency has been observed ...
Page 1-76
... efficiency of bacterial transformation , it is best to set aside a batch of glassware that is used for no other purpose than to prepare competent bacteria . This glassware should be washed and rinsed by hand , filled with pure water ...
... efficiency of bacterial transformation , it is best to set aside a batch of glassware that is used for no other purpose than to prepare competent bacteria . This glassware should be washed and rinsed by hand , filled with pure water ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
96 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml