Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxxii
... Eukaryotic Replicon 16.17 Plasmid DNA Expression Vectors Containing Regulatory Elements from Eukaryotic Viruses 16.17 SIMIAN VIRUS 40 VECTORS 16.17 BOVINE PAPILLOMAVIRUS VECTORS 16.23 EPSTEIN - BARR VIRUS VECTORS Amplification Systems ...
... Eukaryotic Replicon 16.17 Plasmid DNA Expression Vectors Containing Regulatory Elements from Eukaryotic Viruses 16.17 SIMIAN VIRUS 40 VECTORS 16.17 BOVINE PAPILLOMAVIRUS VECTORS 16.23 EPSTEIN - BARR VIRUS VECTORS Amplification Systems ...
Page 3-38
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Treatment of Eukaryotic DNA with Alkaline Phosphatase If the supply of eukaryotic DNA is plentiful , prepare it for cloning by partial digestion and fractionation ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Treatment of Eukaryotic DNA with Alkaline Phosphatase If the supply of eukaryotic DNA is plentiful , prepare it for cloning by partial digestion and fractionation ...
Page 3-40
... eukaryotic DNA . 1. Set up two reaction mixtures as follows : Reaction a fragments of eukaryotic DNA ( 35–45 kb in size ) HindIII / BamHI fragment of pJB8 2 με 1 με 1 με Sall / BamHI fragment of pJB8 10 ligation buffer ( see page 3.29 ...
... eukaryotic DNA . 1. Set up two reaction mixtures as follows : Reaction a fragments of eukaryotic DNA ( 35–45 kb in size ) HindIII / BamHI fragment of pJB8 2 με 1 με 1 με Sall / BamHI fragment of pJB8 10 ligation buffer ( see page 3.29 ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml